Tineke Lenstra Group

Transcription dynamics, single-molecule imaging

Our Focus

Gene expression is a tightly regulated process to ensure that genes are activated in the right cell at the right time. In the last half century, our knowledge of gene regulation has greatly advanced, but the majority of measurements come from large populations of cells. However, individual cells in a population can exhibit considerable variability in transcriptional responses, arising from the random collision of molecules. This stochastic gene expression variation can influence important cell fate decisions and can also contribute to heterogeneity in tumors. To understand the mechanisms and dynamics of gene expression in single cells, our group employs and develops cutting-edge single-molecule microscopy techniques to visualize the behavior of individual protein and RNA molecules in living cells. Our imaging assays are combined with novel gene-specific targeting approaches to modulate transcription of specific genes. By utilizing a combination of single-molecule microscopy, biophysical, genetic and molecular biology approaches in both yeast and mammalian model systems, we aim to understand the molecular mechanisms of transcription regulation, and how stochasticity in transcription modulates cell-to-cell variability and contributes to cancer progression.

Gert-Jan Kuijntjes Phd Student
Heta Patel Phd Student
Ineke Brouwer Post Doc
Jos Meeussen Phd Student
Marit de Kort Phd Student
Simona V. Antonova Post Doc
Wim de Jonge Post Doc
Wim Pomp Research Assistant
Contact Information
Tineke Lenstra Oncode Investigator

More Research

Research Strategy
Focus and Approach
Over 800 scientists working under a single strategy. Oncode is all about uniting efforts and bringing focus to research.
Support Programme
Our supporting programmes
New plans that help fulfil Oncode's objectives get our full support. Young talents are matched with mentors on the basis of their personal endeavours.