Equipment

Location: UMC Utrecht

Contact person: Livio Kleij

Short description: The Spinning Disk Confocal microscope at the UMCU is specifically designed for the imaging of 3D-Cell culture models. Think of organoids, spheroids, zebrafish embryos, or whole organ imaging.

Location: NKI

Contact person: Ron Kerkhoven

Short description: The Illumina NextSeq 500 is a next-generation sequencer that can produce up to 120Gb of sequencing data in a single run. The NextSeq enables rapid and cost-effective sequencing of single-cell libraries. Obvious applications are single-cell RNAseq (including T-cell receptor repertoire mapping) and single-cell copy number variation sequencing to chart the inherent heterogeneity present in tumours.

Location: Radboud University

Contact person: Pascal Jansen

Short description: The Q Exactive HF-X (Thermo) is a so-called High Field (HF) Orbitrap mass spectrometer, which can be used to measure in depth (phospho)proteomes, sub proteomes of cells, or other biological material of interest. Furthermore, researchers can use this instrument for interaction-proteomics studies, for example to investigate protein-protein or protein-DNA interactions.

Location: NKI

Contact person: Martijn van Baalen

Short description: The BD FACSAria™ Fusion SORP is a 6-laser cell sorter that can be used to identify and isolate specific cell subsets from a heterogeneous sample with up to 18 fluorescent reporters. Four populations can be collected simultaneously, but single cell sorting is also possible. The instrument is located inside a biosafety cabinet and can therefore be used to sort BSL2 class organisms and primary human cells.

Location: Erasmus MC

Contact person: Gert van Cappellen

Short description: The Confocal Microscope Leica SP8 AOBS is a DNA damage-dedicated live cell imaging microscope and provides a unique and versatile system capable of inducing virtually all relevant types of DNA lesions. The use of dedicated 266 nm, 355 nm or 405 nm lasers in sub-nuclear areas of living cells, allows to determine the kinetics of multiple DNA damage proteins. DNA damage induction can be automated within the associated operating software.

Location: LUMC

Contact person: Lennard Voortman

Short description: High speed confocal imaging platform, ideal for high speed live-cell imaging. To enable time-lapse imaging, the system is fully motorized and equipped with an incubation chamber.

Location: Amsterdam UMC

Contact person: Jan Paul Medema

Short description: The nanostring nCounter SPRINT is an RNA sequencing technology that provides an easy way to screen for a defined set of RNAs at very low quantity without the need for amplification steps. Importantly, also in samples of relatively poor quality (such as formalin-fixed paraffin-embedded (FFPE) tissue), the Nanostring nCounter SPRINT yields reliable data.

Location: Princess Máxima Center

Contact person: Wilbert Vermeij

Short description: The Seahorse XFe24 Analyzer allows researchers to measure a variety of key metabolic parameters and functions in isolated mitochondria, living cells, organoids, cultured human or mouse tissue slices, and fresh patient material. All in a blinded randomized manner in a 24-well format. It can monitor differential functional metabolism between healthy normal tissue and cancers, as well as their responsiveness to therapy and interventions.

Location: NKI

Contact person: Hans Janssen

Short description: This Electron Microscope is mainly used for single particle analysis of protein complexes under cryo-conditions. Researchers can pre-screen complexes under cryo-conditions for purity and homogeneity, collect images to allow 2D-classification, and obtain the initial 3D-models.

Location: NKI

Contact person: Tineke Lenstra

Short description: With this microscope, the area of ​​interest in a cell can be selected and followed in 3D. This technology makes it possible to follow the location of a specific molecule in time.